https://www.verstegen-lab.org/inside-projects-p20-bu daily 0.75 2024-06-09 https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595084094620-G3B308DANK5J8GSKGK1V/fig%2B2%2Bpos_PAG.jpg inside Projects-P20-bu Figure 2 The Periaqueductal Gray https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595084094630-V78AGUHV4ITTOYH1NRPF/fig+4+neg_hippocampus.jpeg inside Projects-P20-bu Figure 4 Negative activation in the Hippocampus https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595084094616-0F2F1XLL4WS2FUEUOP5N/fig+1+pos_PMC.jpeg inside Projects-P20-bu Figure 1 The PMC https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595084094625-DFW9SS5PQEZI7A8Y1JCI/fig+3+pos_hypothalamus.jpeg inside Projects-P20-bu Figure 3 The Hypothalamus https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595084094634-X2K3CS0W4SQ1I6BOSIOF/fig+5+neg1+hipo_epi.jpeg inside Projects-P20-bu Figure 5 The same as Figure 4 but showing the data in the form of the echo planar image scans - the actual fMRI data https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595084094643-M4CBMRZGJI00CFHENBUZ/April19+rotation.png inside Projects-P20-bu https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595084094639-EHL8HOMWGL6QKGP18PA9/April19+translate.png inside Projects-P20-bu Figure 6. The six possible axes of motion that can be made while in a MRI machine. Less is best with ideally lower than 1mm translation and 1 degree of rotation. https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/20149d63-50c4-4fd6-95bd-43dfc2c37319/Bryce+Mciver_MRI+core_BW_websiteP20.png inside Projects-P20-bu Bryce Maciver, Ph.D. Project Coordinator. Division of Nephrology, Department of Medicine https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/b8d3ac22-146b-4cfa-8258-483bf068958d/Aaron+Grant_MRI+core_rgb_websiteP20.png inside Projects-P20-bu Aaron Grant Ph.D. Director. Preclinical MRI Core at BIDMC https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/c98f9802-3123-4ef4-914c-8d8c7ea8e05b/Mark+Zeidel_headshot_rgb_websiteP20.png inside Projects-P20-bu Mark Zeidel, MD. Principal Investigator. Chairman, Department of Medicine https://www.verstegen-lab.org/verstegen-lab-home daily 1.0 2024-09-06 https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1656162819508-Y5GTZ9UH8YKN4PV8M6AE/CLS2.png Verstegen lab Center for Life Science building https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1599489561180-159GSWUGHWZTGFUPC1Z7/HMS%252Blogo.jpg Verstegen lab https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1599489431019-BSWEV1X2PGO6A5ZSDX77/BIDMC%2Blogo.jpg Verstegen lab https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595084108689-HP505M526ITX21MFDTO4/image-asset.octet-stream Verstegen lab https://www.verstegen-lab.org/ml-publication-list daily 0.75 2020-07-18 https://www.verstegen-lab.org/mvt daily 0.75 2022-08-11 https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595084107025-NJ1SBE0WHUTVFP7WUU9K/11-27-2019_methods_MVT.png MVT MVT imaging setup and enclosure pattern for laser-cut acrylic sheets to produce chamber walls angling slightly outward (to allow full view of the inside corners from above). Void spots along the cage walls and corners can diffuse radially in the dry filter paper underneath the enclosures. https://www.verstegen-lab.org/learn-more-profiling daily 0.75 2025-07-26 https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1599662826724-REGZW226D0JK83PW4WBX/Screen%2BShot%2B2020-09-09%2Bat%2B10.42.43%2BAM.jpg Learn more - Profiling https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/0dce561a-21b0-4181-b721-05f25bee4124/Screen+Shot+2024-01-28+at+11.48.24+AM.png Learn more - Profiling Violin plots showing mean +/- S.E.M. of unique molecular identifiers (UMIs) per cluster. The mean +/- S.E.M. genes detected for each cluster and mitochondrial gene percentage. https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/5bf1db49-e20b-4718-a63f-4ee40ce37082/Screen+Shot+2024-01-28+at+2.41.08+PM.png Learn more - Profiling Dendrogram showing relatedness of glutamatergic neuronal populations in/near Barrington’s nucleus. https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1599662678530-W094EG22VNDN3DAB97UU/Screen+Shot+2020-09-09+at+10.43.48+AM.png Learn more - Profiling https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/37107723-40e2-4a40-af08-03ccd6e7c2d9/Screen+Shot+2024-01-28+at+11.48.55+AM.png Learn more - Profiling Pictured above is the clustering analysis of ~61,000 single cells clustered based on their gene expression profile. The resulting UMAP plot contains 13,900 neurons colored per density clustering, and annotated according to known glutamatergic neuron subtypes in/near Barrington’s nucleus.. https://www.verstegen-lab.org/learn-more-neural-manipulation daily 0.75 2025-07-26 https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595084085555-P8YBQGH5IM6UMMI2G6CN/11-27-2019_cartoon%252BPAGtoPMC_FP_new%252B.jpg Learn more - Neural manipulation Schematic of GCaMP6s construct and viral targeting unilateral to a Barrington’s nucleus-afferent brain site. https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595084085546-3B5BVUWLZ1A27YQ07O3C/11-27-2019_cartoon%25252BPAGtoPMCopto_new%25252B.jpg Learn more - Neural manipulation Schematic of ChR2-mCherry construct and viral targeting unilateral to a Barrington’s nucleus-afferent brain site. https://www.verstegen-lab.org/research-interests daily 0.75 2025-07-26 https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595890421544-0ER3YMCJ6CA8O7F9IIGZ/-sagittal_gallery_neuro+and+thermo_7-27-2020.png Research Interests https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595890432983-GIL74VCRWFXFAEVOM45X/-sagittal_gallery_seq_7-27-2020.png Research Interests https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595890445956-A5Y0X9UCFBBJU3Q2GWKY/-sagittal_gallery_30plus_7-27-2020.png Research Interests https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595890642188-DUXHZ5B36FQ4E2NQ0EHX/-sagittal_gallery_models+no%2C.png Research Interests https://www.verstegen-lab.org/learn-more-mapping-project daily 0.75 2025-07-26 https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595084118215-R0OPKD5JJAZKY1DGR969/-CTb+figure+10+abc_11-26-2019.png Learn more - Mapping project Injecting CTb into the lumbosacral spinal cord retrogradely labels neurons in Barrington’s nucleus and the Locus Coeruleus (LC). https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595084118211-CBX7EX1AW9R6JUPA6FG8/11-26-2019_PMC_vGLUT2_vs_VGAT-GFP.png Learn more - Mapping project Barrington’s nucleus contains predominantly glutamatergic neurons, as shown by the presence of a GFP reporter for the type 2 vesicular glutamate transporter (Vglut2) and absence of a reporter for the vesicular GABA/glycine transporter (Vgat). Panels (a) and (b) compare the distributions of Vglut2 (glutamatergic) and Vgat (GABAergic/ glycinergic) neurons, respectively. https://www.verstegen-lab.org/contact daily 0.75 2022-06-11 https://www.verstegen-lab.org/funding-projects daily 0.75 2024-06-09 https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595879094197-NP4ZTYPMJMBOW0PAGM3E/-11-27-2019_abstract_bladder_meter_brainstem%3F.png Projects https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595888956395-9J77SFW9VCXB7ND936V4/-11-27-2019_cover%2C+methods+MVT_7-27-2020.png Projects Micturition Video Thermography (MVT) setup for recording awake behavior. The setup consists of a FLIR A65 thermal imaging temperature sensor positioned over the center of (up to) four plexiglass behavior boxes atop filter paper. https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/ec8e545d-395e-4f69-ac13-c8f119b34491/P20-fMRI+BIDMC_2019-2021.png Projects - Make it stand out Neuroimaging in the BIDMC Preclinical MRI Core https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1595879059824-5BZJYBFLU0ZE9UUNK0S0/-paper_GA.png Projects https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/a93de634-a3c6-40d8-ae17-b5d7e950318f/Screen+Shot+2024-01-28+at+11.48.55+AM.png Projects https://www.verstegen-lab.org/team daily 0.75 2024-09-17 https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/d03f161b-b13b-40a6-b98f-24c75bec5378/Richard+headshot_v9-6-2024_2.jpg Team - Make it stand out Richard Lee, BS. — Research Assistant rlee21(at)bidmc.harvard.edu https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/9e4e434f-272f-4dd0-90df-5319aaad7a7f/hv1-8-2023_bw_crop+copy.jpg Team Hanneke Verstegen, Ph.D. — Principal Investigator aversteg(at)bidmc.harvard.edu Publication List https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1634937768372-NA34DNJBR48VGCMNWKP1/Natalie.png Team Natalie Klymko, MSc. — Research Associate Sr. nklymko(at)bidmc.harvard.edu Publication List https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/1634938001329-D6HUEYDISEW8YOYAQS9K/Andrea.png Team - Make it stand out Andrea Sartori, Ph.D. — Postdoctoral Fellow asartori(at)bidmc.harvard.edu Publication List https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/18fc2fc7-bb13-440c-8bab-2cf43987e61e/Cassandra+Headshot_v9-6-2024.jpg Team - Make it stand out Cassandra Seifert — Research student MD Candidate at University of Maryland School of Medicine, class of 2025 https://www.verstegen-lab.org/anne-mj-verstegen daily 0.75 2025-02-11 https://images.squarespace-cdn.com/content/v1/5f130d34d1f06d25d242031c/a74274d1-6437-46dc-b703-15650e65f82e/hv1-8-2023_bw_crop+copy.jpg Anne M.J. Verstegen - Hanneke verstegen Assistant Professor of Medicine https://www.verstegen-lab.org/hv-faculty-page-assprof_2020 daily 0.75 2025-07-26 https://www.verstegen-lab.org/ams-publication-list daily 0.75 2021-10-31